Drying paraffin sections on hotplate unadvisable
نویسندگان
چکیده
منابع مشابه
Multiparameter immunofluorescence on paraffin-embedded tissue sections.
Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...
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We perform molecular dynamics simulations of the hydrophobic collapse of two paraffin plates to examine how the collapse is mediated by realistic paraffin-water attractive van der Waals forces. We explore several aspects of the drying transition between the plates, including the critical separation for drying and the critical size of the vapor bubble required for the nucleation of the drying ev...
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The development of the techniques for cutting frozen sections, in particular the use of cryostat microtomes (Pearse, 1960; Cunningham, Bitensky, Chayen, and Silcox, 1960 and 1962; Goodbody, 1963 a and b; Cunningham, 1963), has resulted in sections which not only are produced routinely very rapidly but which are completely acceptable histologically. Such sections would be ideal, especially where...
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Lectins are potentially useful tools in histopathology for the identification of carbohydrates and distinguishing cells according to their type, differentiation or function. Conjugated to fluorescent or enzyme labels, lectins are simple to use on fresh tissue but fixation and processing sequesters glycoconjugates and dissolves out fat-linked sugars. We describe here the use of labelled antibodi...
متن کاملWhole-slide, quadruple immunofluorescence labeling of routinely processed paraffin sections.
Whole-slide images (WSI) have acquired a stable place in diagnostic histopathology and immunohistochemistry. Immunofluorescence (IF) techniques hold a limited and selective role in diagnostics (eg, renal and cutaneous pathology) and so far remain excluded from the digital pathology evolution, with notable exceptions, such as quantitative immunopathology. We explored the ability of a commercial ...
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ژورنال
عنوان ژورنال: Journal of Histology and Histopathology
سال: 2016
ISSN: 2055-091X
DOI: 10.7243/2055-091x-3-4